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5-Aminolevulinic acid hydrochloride

CAS No.5451-09-2
Chemical Name:5-Aminolevulinic acid hydrochloride
CBNumber:CB0496757
Molecular Formula:C5H10ClNO3
Formula Weight:167.59
MOL File:5451-09-2.mol

5-Aminolevulinic acid hydrochloride Property

Melting point ~150 °C (dec.)
bulk density 400kg/m3
Flash point 155-157°C
storage temp. -20°C
solubility H₂O: 50 mg/mL
form powder
color White to pale yellow
PH pH (10g/l, 25℃) : 2.5～3.0
Water Solubility Soluble in dimethyl sulfoxide, methanol and water.
Decomposition 155-157 ºC
Sensitive Hygroscopic
Merck 14,446
BRN 3690651
Stability Hygroscopic
InChIKey ZLHFONARZHCSET-UHFFFAOYSA-N
CAS DataBase Reference 5451-09-2(CAS DataBase Reference)
EWG's Food Scores 1
NCI Dictionary of Cancer Terms aminolevulinic acid hydrochloride; Levulan Kerastick
FDA UNII V35KBM8JGR
NCI Drug Dictionary aminolevulinic acid hydrochloride
UNSPSC Code 41116107
NACRES NA.75

Safety

Hazard Codes :Xi,Xn
Risk Statements :36/37/38-66-20/21/22-36/38
Safety Statements :26-36/37-37/39-36
WGK Germany :3
RTECS :OI1640000
F :3-8-10
Hazard Note :Irritant
HS Code :29224999

NFPA 704:

	1
1		0

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordWarning
Hazard statements H315-H319
Precautionary statements P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313

5-Aminolevulinic acid hydrochloride Price More Price(16)

Brand: Sigma-Aldrich(India)
Product number: A7793
Product name : 5-Aminolevulinic acid hydrochloride
Purity: BioReagent, suitable for cell culture, powder, ≥98%
Packaging: 10MG
Price: ₹2749.55
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: A7793
Product name : 5-Aminolevulinic acid hydrochloride
Purity: BioReagent, suitable for cell culture, powder, ≥98%
Packaging: 500MG
Price: ₹15144.18
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: PHR2993
Product name : Aminolevulinic Acid Hydrochloride
Purity: pharmaceutical secondary standard, certified reference material
Packaging: 500MG
Price: ₹17038.55
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: A7793
Product name : 5-Aminolevulinic acid hydrochloride
Purity: BioReagent, suitable for cell culture, powder, ≥98%
Packaging: 1G
Price: ₹29812.05
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: A3785
Product name : 5-Aminolevulinic acid hydrochloride
Purity: ≥98%
Packaging: 500MG
Price: ₹17547.33
Updated: 2022/06/14
Buy: Buy

5-Aminolevulinic acid hydrochloride Chemical Properties,Usage,Production

Description 5-Aminolevulinic acid hydrochloride (5-ALA) is a naturally occurring amino acid that is an intermediate in the biosynthesis of chlorophyll and haemoglobin. It has anti-inflammatory, antioxidant and antitumour activities, and 5-Aminolevulinic acid hydrochloride-mediated sonodynamic therapy (SDT) has antitumour effects on pancreatic cancer cells. In addition, it is a visualising agent that causes high-grade gliomas to fluoresce under blue light, which can help guide surgeons in removing tumours and is considered a useful imaging tool for brain tumour resection.
Chemical Properties white to pale yellow crystals or
Originator Levulan Kerastick,DUSA Pharmaceuticals Inc.
Uses 5-Aminolevulinic acid hydrochloride has been used as a supplement for culturing Escherichia coli cells for heme biosynthesis.
Uses 5-Aminolevulinic acid hydrochloride finds an important role as a precursor in the synthesis of tetrapyrroles such as chlorophyll and heme. It is widely utilized in photodynamic therapy of diseases namely, Paget?s disease and human papillomavirus (HPV) infection-associated cervical condylomata acuminata.
Uses Naturally occurring amino acid; precursor of tetrapyrroles in the biosynthesis of chlorophyll and heme. Antineoplastic (photosensitizer).
Definition ChEBI: 5-aminolevulinic acid hydrochloride is a hydrochloride that is the monohydrochloride of 5-aminolevulinic acid. It is metabolised to protoporphyrin IX, a photoactive compound which accumulates in the skin. Used in combination with blue light illumination for the treatment of minimally to moderately thick actinic keratosis of the face or scalp. It has a role as an antineoplastic agent, a photosensitizing agent, a dermatologic drug and a prodrug. It contains a 5-ammoniolevulinic acid.
Application 5-Aminolevulinic acid hydrochloride has been used to activate or inhibit heme biosynthesis in HeLa and K562 cell lines. It has also been used in photodynamic therapy in mice with A431 tumours. Clinical oral administration of 5-Aminolevulinic acid hydrochloride potentiates the antihypertensive effects associated with anaesthetics and is used in the treatment of hypertensive patients, particularly in elderly hypertensive patients receiving antihypertensive medications^[1-2].
Manufacturing Process 1) Oxidation Step
2.27 g (10.0 mmol) of N-furfurylphthalimide was charged into a three-necked glass flask equipped with an oxygen feed tube, a thermometer, and a reflux condenser, and dissolved in 100 ml of anhydrous pyridine. After the addition of 7.0 mg of Rose Bengal, oxygen gas was fed at a rate of 20 ml/min at 10°- 20°C under irradiation by light. A 27 W white fluorescent lamp was used as a light source and the radiation was performed from the outside of the flask. After 7 hours, the irradiation was terminated and the pyridine was evaporated under reduced pressure to obtain 2.47 g of a light brown, semi-crystalline product.
2) Reduction Step (Hydrogenation)
2.00 g of the semi-crystalline solid obtained in (1) was dissolved in 40 ml of methanol and stirred at 50°C in a hydrogen atmosphere under atmospheric pressure in the presence of 200 mg of 5% palladium-on-carbon catalyst.
After five hours, the reaction was terminated and the mixture was allowed to cool to room temperature. The catalyst was removed by filtration and methanol was evaporated to obtain 2.11 g of white crystals.
The crystals were identified to be 5-phthalimidolevulinic acid by NMR analysis. The yield was 97%.
3) Hydrolysis Step
100 ml of 6 N hydrochloric acid was added to 2.11 g of the white crystals (2), and the mixture was heated under reflux for 5 hours.
After evaporating the hydrochloric acid under reduced pressure, a brown solid product was obtained and dissolved in ethanol. Acetone was added to the solution and the crystals produced were collected by filtration to obtain 0.689 g of 5-aminolevulinic acid hydrochloride. The yield based on Nfurfurylphthalimide was 51%.
NMR spectrum data conformed to 5-aminolevulinic acid hydrochloride
Therapeutic Function Photosensitizer
Biochem/physiol Actions 5-Aminolevulinic acid (5-ALA) is an intermediate in heme biosynthesis and is useful in cancer treatment. It is a non-protein amino acid. 5-ALA also has applications in the field of agriculture. It is being studied as an inducing reagent for protoporphyrin IX (PPIX) dependent fluorescence diagnosis of metastatic lymph nodes. 5-ALA is used for photodynamic therapy of diseases, such as Paget′s disease and HPV infection-associated cervical condylomata acuminata.
Purification Methods Dry ALA-HCl in a vacuum desiccator over P2O5 overnight, then crystallise it by dissolving it in cold EtOH and adding dry Et2O. Also crystallis
References [1] Oral Aminolevulinic Acid Hydrochloride[J]. Definitions, 2020. DOI:10.32388/vh56ni.
[2] NOBORU FUKUDA . 5-Aminolevulinic acid hydrochloride enhances bupivacaine-induced hypotension in spontaneously hypertensive rats[J]. Journal of pharmacological sciences, 2023. DOI:10.1016/j.jphs.2023.02.007.