Purification Methods
It is now available commercially. After the sequential removal of C60 and C7 0 fullerenes from soot extracts (see above) on gel permeation columns (e.g. Buckyclutcher 1 column), C7 6 and higher fullerenes are obtained . These are further separated on a Trident-TriDNP functionalised silica column. After two HPLC runs on a C1 8 reverse phase (Vydac 201 TP C1 8) column and eluting with 1:1 MeCN/toluene, pure C7 6 fullerene is obtained. The identity is confirmed by HPLC/GPC system with Waters 600E UV/VIS detection, mass and NMR spectroscopy. [Seleque et al. In Kadish and Ruoff (Eds) Fullerenes: Recent Advances in the Chemistry and Physics of Fullerenes and Related Materials The Electrochemical Soc. Inc, Pennington, NJ, 1994 ISBN 1566770823, Diederich & Whetten Acc Chem Res 25 119 1992, Diederich et al. Science 254 1768 1991.]
