Uses
BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5to 30g/mL in tube assays and 1to 20g/mL in 96- or 384-well plate assays. It is used for studying protein: protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.
Description
The BCA Protein Assay Kit can be used to measure the protein concentration of lysates or homogenates, in microplate format, prepared with the following buffers: Cell Lysis Buffer (10X)
Background
Bicinchoninic Acid is capable of forming an intense purple complex with cuprous ion, Cu1+, in an alkaline environment. Cu1h+ is produced from the reaction of protein with alkaline Cu2+. The resulting reaction and color produced is the basis for a common protein quantification method capable of measuring protein concentration over a wide range. Increasing protein concentrations produce proportionally deeper colors. The BCA protein assay demonstrates higher tolerances towards common interfering substances, such as nonionic detergents and buffer salts, than the Lowry technique.
References
[1] Smith, P.K. et al. (1985) Anal Biochem 150, 76-85.
