Chemical Properties
dark blue-violet-brown crystalline powder
Uses
Brilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.
Preparation
Benzaldehyde ?(1 Moore) and N-ethyl-N-benzyl-3-methylaniline?(2 Moore) condensation, And then sulfonating the product of three acids, and lead oxide, peroxide, and then the reaction with the 4-Ethoxybenzenamine, Benzaldehyde ?sulfonic acid groups on the phenyl ring is substituted anilino-ethoxy.
General Description
Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.
Flammability and Explosibility
Not classified
Biological Activity
brilliant blue g is also a selective inhibitor of the p2x purinoceptor channel p2x7 [1].p2x receptors are membrane ion channels activated in response to the binding of extracellular atp. seven p2x subtypes have been identified. p2x receptors have been involved in in diverse patho- and physiological processes, such as the autonomic nervous system, afferent signalling, chronic pain, and in autocrine loops of endothelial and epithelial cells. the p2x7 receptor plays a prominent role in certain neurologic disorders, such as ischemia-reperfusion injury, alzheimer's disease, spinal cord injury and sensory neuropathies [2].in hek293 cells heterologously expressing human p2x7 receptors, brilliant blue g noncompetitively inhibited rat and human p2x7 receptors with ic50 values of 10 and 200 nm, respectively. the ic50 values for inhibition of the other p2x receptors ranged from 2 to >30 μm; brilliant blue g inhibited the rat and human p2x4 receptors with the ic50 values of >10 and 3.2 μm [1]. subretinal injection of bbg caused retinal cell degeneration at lower concentrations. subretinal injection of bbg (0.25 mg/ml) provided satisfactory biocompatibility [3].
Biochem/physiol Actions
P2X7 purinergic receptor antagonist.
Properties and Applications
bright blue. Dark brown powder, slightly soluble in water, soluble in hot water (bright blue) and ethanol (bright blue). When a blood-red of sulfuric acid, a orange red after diluted. Its water solution and sodium hydroxide for purple. Used for wool and silk dyeing, also can be used for leather dyeing.
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Standard
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Light Fastness
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Soaping
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Persperation Fastness
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Oxygen bleaching
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Fastness to seawater
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Fading
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Stain
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Fading
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Stain
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Fading
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Stain
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ISO
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3
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3
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4-5
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3-4
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1
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1
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4
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4
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AATCC
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3
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3-4
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3-4
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4-5
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1
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1
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4-5
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4-5
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References
[1] jiang l h, mackenzie a b, north r a, et al. brilliant blue g selectively blocks atp-gated rat p2x7 receptors[j]. molecular pharmacology, 2000, 58(1): 82-88.
[2] sperlágh b, vizi e s, wirkner k, et al. p2x 7 receptors in the nervous system[j]. progress in neurobiology, 2006, 78(6): 327-346.
[3] ueno a, hisatomi t, enaida h, et al. biocompatibility of brilliant blue g in a rat model of subretinal injection[j]. retina, 2007, 27(4): 499-504.
