ANTI-HUMAN MIP-1ALPHA

Uses

Anti-MAPKAP1/SIN1 antibody is suitable for use in immunoprecipitation (5-10 μg using lysates of mouse C2C12 cells) and western blot (0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human MAPKAP1).

Uses

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit .

General Description

Mitogen-activated protein kinase associated protein 1 (MAPKAP1 )/stress-activated protein kinase-interacting protein 1 (SIN1) is an essential subunit of the mammalian target of rapamycin complex 2 (mTORC2) complex.

Biochem/physiol Actions

Mitogen-activated protein kinase associated protein 1 (MAPKAP1 )/stress-activated protein kinase-interacting protein 1 (SIN1) regulates cell survival and cytoskeletal organization. It maintains mammalian target of rapamycin complex 2 (mTORC2) integrity and modulates Akt phosphorylation. Knockdown of SIN1 results in the disruption of the rictor-mTOR interaction and in defective Akt-Ser473 phosphorylation.