HISTONE

Uses

Histone H3 has been used to determine the role of histones in metastasis. It has also been used in dot blot and western blotting.

General Description

Mixture of histones H1, H2A, H2B, H3, and H4, isolated from calf thymus.
Histones are a group of DNA-binding proteins that are characterized by relatively high levels of lysine and arginine. Five different fractions of histones have been isolated and characterized. These are named as H1, H2A, H2B, H3 and H4. The H1 fraction is lysine-rich, while the H2A and H2B are slightly lysine-rich, The H3 and H4 fractions are arginine-rich. The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction.

Biochem/physiol Actions

Histone proteins are basic building blocks of chromatin. Mutations in Histone H3 results in adult cerebellar high-grade gliomas. It controls protein-protein interactions to induce binding of trans-acting factors that “drive” chromatin condensation. H3.1 acts as a replication-dependent histone.