Description
15-deoxy-Δ
12,14-PGD
2 is a metabolite of PGD
2 . It is an agonist of PGD
2 receptor 2 (DP
2) that binds DP
2 (K
i = 50 nM for the mouse receptor expressed in HEK293 cell membranes) and induces activation of eosinophils (EC
50 = 8 nM). It also stimulates the recruitment of steroid receptor coactivator-1 (SRC-1) to peroxisome proliferator-activated receptor γ (PPARγ) and induces PPARγ-mediated transcription in a reporter assay when used at a concentration of 5 μM. 15-deoxy-Δ
12,14-PGD
2 is cytotoxic to L1210 murine leukemia cells (IC
50 = 0.3 μg/ml). It inhibits ADP-induced platelet aggregation (IC
50 = 320 ng/ml) less potently than PGD
2.