Uses
NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity[1].
Biological Activity
Cell permeable: no', 'Primary Target
Matrix Metalloproteinases (MMPs)', 'Product does not compete with ATP.', 'Reversible: no', 'kcat/Km = 5.9 x 104 M-1sec-1 as substrates for MMP-3 (stromelysin-1)
References
[1] Giricz O, et al. Comparison of metalloproteinase protein and activity profiling. Anal Biochem. 2011 Feb 1;409(1):37-45. DOI:
10.1016/j.ab.2010.09.040