Description
10-
Pyrene-
PC is a substrate for all PLA
2s with the exception of cPLA
2 and PAF-AH. Upon phospholipid hydrolysis, 10-pyrenyldecanoic acid is produced from 10-pyrene-PC. The monomeric 10-pyrenyldecanoic acid exhibits fluorescence (excitation 345 nm, emission 395 nm), allowing quantitation of phospholipase activity.
Uses
1-Palmitoyl-2-pyrenedecanoylphosphatidylcholine is a fluorescent phospholipid used to study the mechanism of spontaneous phospholipid transfer between single-walled phospholipid vesicles (liposomes) and other membrane interactions.
References
[1] ROBERTS M F. Phospholipases: structural and functional motifs for working at an interface[J]. FASEB Journal, 1996, 10 10: 1159-1172. DOI:
10.1096/fasebj.10.10.8751718[2] S LUSA. Degradation of pyrene-labelled phospholipids by lysosomal phospholipases in vitro. Dependence of degradation on the length and position of the labelled and unlabelled acyl chains.[J]. Biochemical Journal, 1996, 315 ( Pt 3): 947-952. DOI:
10.1042/bj3150947
Excitation / Emission Maximum
342 nm/376 nm
