LUCIFER YELLOW CH DILITHIUM SALT

Description

Lucifer Yellow CH is a highly sensitive yellow fluorescent dye widely used in neurobiology, cytology, and molecular biology for cell tracing, studying cell-cell interactions, and visualizing neurons. The dye is suitable for labeling both live and fixed preparations: it has low cytotoxicity, is retained in cells after fixation (e.g., with paraformaldehyde), and is compatible with most immunochemistry protocols. Lucifer Yellow CH has a high quantum yield, is resistant to photobleaching, and is, therefore, ideal for confocal and fluorescence microscopy.

The main dye application:

Working dye concentration: 1–5% (depending on protocol).

Chemical Properties

ORANGE POWDER

Uses

Lucifer Yellow CH dilithium salt is a highly fluorescent dye, useful in marking nerve cells. It can be bound to tissue by conventional aldehyde-containing biological fixatives.

Uses

Lucifer Yellow CH dilithium salt is a highly fluorescent dye. It can also be used as reactant/reagent in synthetic preparation of singlet oxygen-induced furan oxidation for site-specific and chemoselective peptide ligation. Dyes and metabolites.

Definition

ChEBI: An organic dilithium salt having 6-amino-2-[(hydrazinocarbonyl)amino]-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinoline-5,8-disulfonate as the counterion.

Abs/Em Maxima

428/533 nm

Excitation / Emission Maximum

430 nm/540 nm