Uses
Lysostaphin from
Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of
Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a
Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity.
Biochem/physiol Actions
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5
