Uses
Component of NADH recycling systems. Substrate specificity limited to low molecular weight alcohols.
General Description
Alcohol:NAD
+ oxidoreductase.
Alcohol dehydrogenase (ADH) from yeast is a metalloenzyme containing four zinc atoms per molecule. NAD
+ is its coenzyme.
Biochem/physiol Actions
Alcohol dehydrogenase catalyzes the oxidative conversion of alcohol into aldehyde. It has a homodimeric structure with a co-enzyme binding domain at the C-terminal and an N-terminal catalytic domain. The active site is located at the interdomain cleft. Binding of NAD+ in the active site causes conformational changes which create the binding site for the alcohol substrate.
