ALPHA-ACTININ Chemical Properties,Usage,Production
Uses
α-Actinin from chicken gizzard has been used:
as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones
in in vitro motility experiments to test its effect on actin filament movement
to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies
General Description
α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures.
Biochem/physiol Actions
α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle.
