Chemical Properties
Off white to tan crystals
Uses
Suitable for visualizing protein-peroxidase conjugates in western blotting.
Uses
4-Chloro-1-naphthol has been used to visualize protein bands in western blotting. It has also been used in staining polyvinylidene fluoride (PVDF) membrane by Nakane method.
General Description
4-Chloro-1-naphthol is primarily used for the detection of proteins, it gives a characteristic purple precipitate and the reactions are easily controllable. It can be used as a substitute for benzidine compounds, which are considered to be carcinogenic.
Biochem/physiol Actions
4-Chloro-1-naphthol serves as a substrate for horseradish peroxidase.
Purification Methods
Crystallise the naphthol from EtOH or CHCl3. [Beilstein 6 H 611, 6 II 582, 6 III 2933, 6 IV 4233.]
