Heterophyllin B (0-200 μM; 12-48 hours) reduces cell viability in the 75, 100 and 200 μM groups, has no effect on 5, 10, 25 and 50 μM groups.Heterophyllin B (0 -50 μM; 6 hours) suppresses the expression levels of p-PI3K, p-AKT and β-catenin in a dose-dependent manner in ECA-109 cells.
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Cell Viability Assay
| Cell Line: | ECA-109 cells |
| Concentration: | 5 μM, 10 μM, 25 μM, 50 μM, 75 μM, 100 μM and 200 μM |
| Incubation Time: | 12, 24 and 48 hours |
| Result: | Decrease cell viability at high concentrations. |
Western Blot Analysis
| Cell Line: | ECA-109 cells |
| Concentration: | 0 μM, 10 μM, 25 μM, 50 μM |
| Incubation Time: | 6 hours |
| Result: | Decreased the phosphorylation of PI3K/AKT and the expression of β-catenin. |
