-N7-DGTP

Uses

7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).

General Description

7-Deaza-2′-deoxyguanosine 5′-triphosphate enhances polymerase chain reaction (PCR) product yield.

Biochem/physiol Actions

This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA ploymerases.