Description
DYKDDDDK peptide is a synthetic octapeptide and fusion tag.1,2 It can be added to the N- or C-terminus of proteins or inserted into the protein sequence and later cleaved using enteropeptidase. Due to its hydrophilicity, it has a low potential to interfere with protein folding or activity. DYKDDDDK peptide has commonly been used as an epitope tag on recombinant proteins to facilitate their detection and purification by DYKDDDDK-specific antibodies in vitro and in vivo.1,2,3,4WARNING This product is not for human or veterinary use.
References
[1] THOMAS P. HOPP. A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification[J]. Nature biotechnology, 1988, 6 10: 1204-1210. DOI:
10.1038/nbt1088-1204[2] A. EINHAUER A. J. The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins[J]. Journal of biochemical and biophysical methods, 2001, 49 1: Pages 455-465. DOI:
10.1016/s0165-022x(01)00213-5[3] TAKAKO ICHIKI. Modulation of leukotriene B4 receptor 1 signaling by receptor for advanced glycation end products (RAGE)[J]. FASEB Journal, 2016, 30 5: 1811-1822. DOI:
10.1096/fj.201500117[4] KOKI IKEDA . Generation and characterization of a human-mouse chimeric high-affinity antibody that detects the DYKDDDDK FLAG peptide[J]. Biochemical and biophysical research communications, 2017, 486 4: Pages 1077-1082. DOI:
10.1016/j.bbrc.2017.03.165
