Description
Reactive oxygen species (ROS) play important roles in the initiation and progression of many disease processes. Most fluorescent probes for ROS detection, such as 2,7-
dichlorofluorescein (DCFH), function by an oxidative mechanism and are useful for total oxidant detection but are not selective for hydrogen peroxide specifically.
1 Pentafluorobenzenesulfonyl fluorescein is a fluorescent probe that functions by a nonoxidative mechanism. It fluoresces upon perhydrolysis of the sulfonyl linkage, and is selective for hydrogen peroxide over hydroxyl radical,
tert-
butyl hydroperoxide, superoxide anion, singlet oxygen, and nitrates. Pentafluorobenzenesulfonyl fluorescein can be used as a probe for hydrogen peroxide in whole cell systems.
1
References
1. Maeda, H., Fukuyasu, Y., Yoshida, S., et al.
Fluorescent probes for hydrogen peroxide based on a non-oxidative mechanism Angew. Chem. Int. Ed. Engl. 43(18),2389-2391(2004).
