Uses
Fumarase from porcine heart has been used:
- as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
- for immobilized enzyme based stability assay
- in fumarate hydratase assays in human lung carcinoma cells (A549)
Definition
An enzyme that catalyzes the interconversion of
fumaric acid and malic acid. It is an important reactant in the tricarboxylic acid cycle.
General Description
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.
Biochem/physiol Actions
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
