Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*

Uses

For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.

• Biotin-16-dUTP is used as asubstrate for: Terminal Transferase
• DNA polymerase I (holoenzyme and Klenow fragment)
• Taq DNA polymerase
• Reverse transcriptase (e.g., Transcriptor)

Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling.

• Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate
• Biotin Luminescence Detection Kit

General Description

Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.

Biochem/physiol Actions

Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.