Uses
Biotin-labeled RNA can be used as a hybridization probe for:
- Northern blots
- Southern blots
- Plaque or colony lifts
- RNase protection experiments
- In situ hybridization
- Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
General Description
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the
in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is
in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
