Uses
Z-Arg-Leu-Arg-Gly-Gly-AMC is a peptide substrate of SARS-CoV PLpro[1].
Biological Activity
Z-RLRGG-AMC is a fluorogenic substrate for the MERS-CoV, SARS-CoV, SARS-CoV-2 papain-like protease (PLpros), deubiquitinating enzyme isopeptidase T (IPaseT) and other ubiquitin C-terminal hydrolases, which consists of the Ub and ISG15 C-terminal sequences. Z-RLRGG-AMC excitation wavelength is 340 nm, and emission wavelength is 460 nm.
References
[1] Schalk F, et, al. GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B. RSC Adv. 2021 May 24;11(31):18748-18756. DOI:
10.1039/d1ra00997d[2] ROSS L. STEIN Francesco M Zhijian Chen. Kinetic studies of isopeptidase T: modulation of peptidase activity by ubiquitin[J]. Biochemistry Biochemistry, 1995, 34 39: 12616-12623. DOI:
10.1021/bi00039a017