Description
Bisphosphonates bind strongly to bone and reduce bone resorption through effects on both osteoclasts and osteoblasts. Clodronate is a non-nitrogenous bisphosphonate that has been used in the treatment of osteoporosis and other metabolic bone diseases. It binds to hydroxyapatite crystals with an affinity constant of 0.72 μM, inhibiting their growth (IC
50 = 1.34 μM) and bone resorption. Clondronate is metabolized intracellularly to a β-γ-methylene analog of ATP that is cytotoxic to both osteoclasts and macrophages, which belong to the same cell lineage as osteoclasts. Liposome-encapsulated bisphosphonates such as clodronate have been used in biological research to selectively deplete macrophage-like cells and cancer cells.
Uses
Disodium Clodronate Tetrahydrate is a bisphosphonate bone growth regulator.
Definition
ChEBI: The tetrahydrate of the disodium salt of clodronic acid. It inhibits bone resorption and soft tissue calcification, and is used as an adjunct in the treatment of severe hypercalcaemia associated with malignancy, and in the management of osteolytic lesions
nd bone pain associated with skeletal metastases.
in vivo
Clodronate disodium tetrahydrate (10 mg/kg; i.v.) attenuates inflammatory pain via VNUT inhibition[1].
Clodronate disodium tetrahydrate attenuates inflammation via VNUT inhibition [1].
| Animal Model: | C57BL/6 mice (22-30 g)[1] |
| Dosage: | 10 mg/kg |
| Administration: | Intravenous injection |
| Result: | Attenuated carrageenan- or complete Freund’s adjuvant (CFA)-evoked inflammatory pain. |
References
[1] G L PLOSKER K L G. Clodronate. A review of its pharmacological properties and therapeutic efficacy in resorptive bone disease.[J]. Drugs, 1994, 47 6: 945-982. DOI:
10.2165/00003495-199447060-00007[2] P. LEHENKARI. Further insight into mechanism of action of clodronate: inhibition of mitochondrial ADP/ATP translocase by a nonhydrolyzable, adenine-containing metabolite.[J]. Molecular Pharmacology, 2002, 61 5 1: 1255-1262. DOI:
10.1124/mol.61.5.1255
