Uses
Monazomycin is a macrocyclic polyol lactone antibiotic isolated from Streptoverticilium.
Uses
Monazomycin is a macrocylic polyol lactone isolated from several species of Streptoverticillium, first reported in 1963. Monazomycin is active against Gram positive bacteria with weak Gram negative activity. In solution, monazomycin exists as hydrophilic clusters which, when adsorbed onto a lipid bilayer, can induce channel formation. Monazomycin is an important bioprobe for understanding membrane channels.
Biological Activity
monazomycin is a positively charged and polyenelike antibiotic produced by streptomyces [1].monazomycin is able to induce a voltage-dependent conductance in lipid bilayer membranes. monazomycin is selective for monovalent cations and can alter the membrane conductance when applied to one or both sides of the membrane [1]. application of micromolar amounts of monazomycin on one side of phospholipid bilayer membranes induced dramatic voltage-dependent conductance effects. the steady-state conductance was proportional to the 5th power of the monazomycin concentration and increased exponentially with positive voltage (monazomycin side positive); there was an e-fold change in conductance per 4–6 mv. the major current-carrying ions were univalent cations. monazomycin monomers cooperated to form a multimolecular conductance channel. the voltage control of conductance arose from the electric field driving monazomycin molecules at the membrane surface into the membrane and thus affecting the number of channels that were formed [2].
References
[1] bamberg e, janko k. single channel conductance at lipid bilayer membranes in presence of monazomycin[j]. biochimica et biophysica acta (bba)-biomembranes, 1976, 426(3): 447-450.
[2] muller r u, finkelstein a. voltage-dependent conductance induced in thin lipid membranes by monazomycin[j]. the journal of general physiology, 1972, 60(3): 263-284.
