Description
Thymosin β4 is an actin-sequestering peptide that has wound healing and anti-inflammatory activities. It regulates cytoskeletal rearrangement by binding to actin (K
ds = 0.7 and 2.1 μM for human platelets and rabbit muscle, respectively) and inhibiting the polymerization of actin monomers. Thymosin β4 increases
in vitro migration of human umbilical vein endothelial cells (HUVECs), keratinocytes, and coronary artery endothelial cells, but not fibroblasts, actin smooth muscle cells, neutrophils, monocytes, or HT-1080 fibrosarcoma cells, when used at concentrations ranging from 0.001 to 1 μg/ml. It increases gap closure and reduces wound width in a rat full-thickness cutaneous wound model when administered at a dose of 60 μg. Thymosin β4 (1 μg/ml) inhibits TNF-α-induced NF-ĸB phosphorylation and nuclear translocation in immortalized human corneal epithelial cells and decreases the expression of chemokine (C-X-C motif) ligand 1 (CXCL1), CXCL2, matrix metalloproteinase-9 (MMP-9), and MMP-2 in the cornea in a mouse model of sodium hydroxide-induced corneal injury when administered at a dose of 5 μg.
References
[1] A. WEBER. Interaction of thymosin .beta.4 with muscle and platelet actin: implications for actin sequestration in resting platelets[J]. Biochemistry Biochemistry, 1992, 31 27: 6179-6185. DOI:
10.1021/bi00142a002[2] KATHERINE M. MALINDA. Thymosin β4 Accelerates Wound Healing[J]. Journal of Investigative Dermatology, 1999, 113 3: Pages 364-368. DOI:
10.1046/j.1523-1747.1999.00708.x[3] GABRIEL SOSNE . Thymosin beta 4 suppression of corneal NFκB: A potential anti-inflammatory pathway[J]. Experimental eye research, 2007, 84 4: Pages 663-669. DOI:
10.1016/j.exer.2006.12.004[4] KATHERINE M. MALINDA;Hynda K K;Allan L Goldstein. Thymosin β4 stimulates directional migration of human umbilical vein endothelial cells[J]. FASEB Journal, 1997, 11 6: 474-481. DOI:
10.1096/fasebj.11.6.9194528
