CK-0157869

Description

CK 869 is an inhibitor of actin polymerization via inhibition of the actin-related protein 2/3 (Arp2/3) complex, leading to reduced cell motility rate and rearrangement of F-actin in M-1 cells. It inhibits comet tail formation by L. monocytogenes with an IC₅₀ value of 7 μM. It also impairs cell adhesion and cell spreading in MCF10A human breast epithelial cells.

Uses

CK-869 binds to the Arp2/3 complex and inhibits nucleation. The Arp2/3 complex is involved with cell motility and F-actin reorganization in M-1 cells.

Uses

CK-869 has been used as an Arp2/3 complex inhibitor:

• to treat cultured mammalian cells and study its in vitro and in vivo effects on microtubule assembly
• to induce membrane blebbing in HT1080 fibrosarcoma cells
• to treat KDM3A-null cells and study the relation between actin dynamics and ciliogenesis

Biochem/physiol Actions

CK-0157869 (CK-869) prevents microtubule (MT) assembly, free of Arp2/3 complex.