Description
TO-TAP-1 (Thiazole Orange Monomer, also known as TO-PRO®-1) is a green fluorescent carbocyanine monomeric dye. TO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.
The bright fluorescence signal and low background make TO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. TO-TAP-1 is non-cytotoxic and may be used for long-term monitoring of cell viability and dead cell detection in culture.
Chemical Properties
Appearance: Green solution
ex/em: 515/531 nm
Extinction coefficient (ε): 62800 cm1M1
Quantum yield (Φ): 0.25
Uses
TO-PRO 1 is a DNA binding fluorochrome, that atached to the surface of the Feraheme (FH) nanoparticle (NP), to obtain a fluorochrome-functionalized NP. TO-PRO 1 binds DNA through intercalation, and acts as a vital fluorochrome for necrotic cells[1].
Definition
ChEBI: A C1 cyanine dye having benzothiazolium-2-yl and quinolinium-4-yl substituents.
References
[1] Hoonsung Cho, et al. Fluorochrome-functionalized nanoparticles for imaging DNA in biological systems. ACS Nano. 2013 Mar 26;7(3):2032-41. DOI:
10.1021/nn305962n
![3-Methyl-2-([1-[3-(trimethylammonio)propyl]-4(1H)-quinolinylidene]methyl)-1,3-benzothiazol-3-ium diiodide Structure](https://www.chemicalbook.com/CAS/GIF/157199-59-2.gif)