4H-Imidazol-4-one, 3-(1H-benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-, (5Z)-

Biological Activity

Cell-permeable DFHBI derivative th at selectively binds to Broccoli aptamer; used for imaging of RNA in living cells.



BI is a cell-permeable DFHBI benzimidazolo (DFHBI-BI) derivative th at greatly and selectively promotes and stabilizes Broccoli aptamer folding in vitro and cells with enhanced photostability. BI displays little background in vitro and cells (ϕ=0.00048, ϵ=42,500 M-1 cm-1), but very high fluorescence activation after binding to Broccoli (ϕ=0.669, ϵ=33,600 M-1 cm-1, KD = 51 nM). DFHBI-BI shows negligible cellular toxicity and binds Squash with much weaker affinity than Broccoli. Ex/Em: 470/505 nm.