2561482-42-4

Cell-permeable DFHBI derivative th at selectively binds to Broccoli aptamer; used for imaging of RNA in living cells.



BI is a cell-permeable DFHBI benzimidazolo (DFHBI-BI) derivative th at greatly and selectively promotes and stabilizes Broccoli aptamer folding in vitro and cells with enhanced photostability. BI displays little background in vitro and cells (ϕ=0.00048ϵ=42,500 M-1 cm-1)but very high fluorescence activation after binding to Broccoli (ϕ=0.669ϵ=33,600 M-1 cm-1KD = 51 nM). DFHBI-BI shows negligible cellular toxicity and binds Squash with much weaker affinity than Broccoli. Ex/Em: 470/505 nm.