APNA

Description

Arachidonoyl p-nitroaniline (ApNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity. FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide (AEA; ). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. (See also Decanoyl p-Nitroaniline - Catalog No. 90349). Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer.

Uses

Arachidonoyl p-Nitroaniline is a nitroaniline probe for FAAH activity.

References

[1] MATTHEW P. PATRICELLI  Benjamin F C. Characterization and Manipulation of the Acyl Chain Selectivity of Fatty Acid Amide Hydrolase[J]. Biochemistry Biochemistry, 2001, 40 20: 6107-6115. DOI: 10.1021/bi002578r