Description
D-Luciferin 1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester is an analog of Luciferin, which is cell-permeable. Upon entering the cell, this compound can be hydrolyzed to D-luciferin generating fluorescence.
Uses
DMNPE-caged D-luciferin is a heterocyclic luminescent compound that is a natural ligand for luciferase, an enzyme used to detect cell activity. Its reaction requires ATP and emits yellow-green light with a peak wavelength of about 530 nm. The luciferin in the DMNPE cage easily crosses the cell membrane.
References
[1] Luque-Ortega JR, et al.
