Chemical Properties
slightly pinkish-beige powder
Uses
Bathophenanthrolinedisulfonic acid disodium salt trihydrate was used in double electrochemical covalent coupling method for the fabrication of sensitive amperometric immunosensor, which was based on electro-click chemistry and diazonium chemistry. It was also used in an experimental study based upon, correlating the iron content with the activity, preparation and reconstituting the apoenzyme phenylamine hydroxylase.
Uses
Bathophenanthrolinedisulfonic acid disodium salt hydrate has been used to measure iron to determine the solubility of iron in the biological sample. It has been used as an iron chelator to grow plants without Fe-EDTA (ethylenediaminetetraacetic acid).
Definition
ChEBI: An organic sodium salt having 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate as the counterion.
General Description
Bathophenanthrolinedisulfonic acid disodium salt forms water soluble complexes with palladium and their catalytic role in oxidation of 2-hexanol has been examined.
Biochem/physiol Actions
Bathophenanthrolinedisulfonic acid is a metal chelator. It permits iron determination to be made in aqueous medium without need for solvent extraction. It forms a complex with Fe2+ and makes it unavailable to Fe2+ transporter, thereby inhibiting the transport of iron into chloroplasts. Bathophenanthrolinedisulfonic acid is also used in the assay for determining intracellular glutathione.
Purification Methods
Dissolve crude sample in the minimum volume of water and add EtOH to precipitate the contaminants. Carefully evaporate the filtrate to obtain pure material. It forms a dark red complex with Fe2+ with 535nm ( 2.23 x 104mol-cm -1) [Imasaka et al. Anal Chim Acta 115 407 1980]. It is prepared by sulfonating bathophenanthroline with ClSO3H: to 100g of bathophenanthroline is added 0.5mL of Fe free ClSO3H and heated over a flame for 30seconds. Cool and carefully add 10mL of pure distilled H2O and warm on a water bath with stirring till all solid dissolves. A stock solution is made by diluting 3mL of this reagent to 100mL with 45% aqueous NaOAc, filter off the solid and store in a dark bottle. In this way it is stable for several months. [Landers & Bennie Am J Clinical Pathology 29 590 1958.]
