Description
24(S),25-epoxy Cholesterol is an oxysterol and the most abundant oxysterol in mouse ventral midbrain. It activates liver X receptors (LXRs) in the developing ventral midbrain to induce stem cell differentiation into dopaminergic neurons. 24(S),25-epoxy Cholesterol inhibits IL-6 production and degranulation of bone marrow-derived murine mast cells that express LXRβ. It inhibits the conversion of desmosterol to cholesterol by 3β-hydroxysterol Δ
24-reductase (DHCR24/Seladin-1) in CHO-7 and SRD-1 cells when used at a concentration of 2.5 μM.
Uses
(24S)-24,25-Epoxycholesterol is a derivative of 4β-Hydroxy Cholesterol (H917980), a metabolite of Cholesterol. It is formed from Cholesterol by the drug-metabolizing enzyme cytochrome P 450 3A4. A potential ligand for the nuclear receptor LXR and also a new endogenous CYP3A marker.
Definition
ChEBI: A 3beta-hydroxy steroid that is desmosterol in which the double bond at position 24-25 has been oxidised to the corresponding epoxide (the 24S diastereoisomer). It is an oxysterol agonist of the liver X receptor.
in vivo
24S,25-Epoxycholesterol (5 mM, icv) enhances the mDA neurogenesis, limits the neurodegeneration in CYP46A1-overexpressing mice midbrain[4].
| Animal Model: | GGPP induced mDA neurogenesis defect in CYP46A1 overexpressing CD-1 mice[4] |
| Dosage: | 5 mM |
| Administration: | icv, 1 μL, single dosage |
| Result: | Increased levels of mDA neurons, blocked the GGPP induced decrease of double EdU and TH cells. |
References
[1] AJAY CHAWLA. Nuclear Receptors and Lipid Physiology: Opening the X-Files[J]. Science, 2001, 294 5548. DOI:
10.1126/science.294.5548.1866[2] BROWN A J. 24(S),25-Epoxycholesterol: A messenger for cholesterol homeostasis[J]. International Journal of Biochemistry & Cell Biology, 2009, 41 4: Pages 744-747. DOI:
10.1016/j.biocel.2008.05.029[3] SPYRIDON THEOFILOPOULOS. Brain endogenous liver X receptor ligands selectively promote midbrain neurogenesis[J]. Nature chemical biology, 2012, 9 2: 126-133. DOI:
10.1038/nchembio.1156
