Description
Di-8-ANEPPS is a potentiometric fluorescent dye that can be used to noninvasively measure transmembrane voltage. It is not fluorescent in water, but when bound to the lipid bilayer, becomes strongly fluorescent (ex/em max = ~467/631 nm). The fluorescence intensity of di-8-ANEPPS varies proportionally to changes in transmembrane voltage. This dye is most suitable for measuring larger changes in membrane voltage, such as the onset of induced membrane voltage in nonexcitable cells exposed to external electric fields or action potentials in excitable cells. Since di-8-ANEPPS stains the membrane, it can also be used simply as a cell membrane marker.
Chemical Properties
Appearance: Orange-red solid
ex/em: 468/633 nm (artificial membrane)
Extinction coefficient (ε): 44,000 Lmol1cm1 (methanol)
Uses
Di-8-ANEPPS is a fast-responding membrane potential dye. It is more suitable for long-term membrane potential studies than di-4-ANEPPS (sc-214872) because of its better retention in the outer leaflet of plasma membranes. This dye is weakly fluorescent in aqueous media, and becomes strongly fluorescent upon binding to lipophilic environments (such as membranes). Di-8-ANEPPS is less susceptible for cellular internalization due to the sulfonate group it possesses.Dyes and metabolites.
Uses
Di-8-ANEPPS is a potentiometric fluorescent dye that can be used to noninvasively measure transmembrane voltage. It is not fluorescent in water, but when bound to the lipid bilayer, becomes strongly fluorescent (ex/em max = ~467/631 nm). The fluorescence intensity of di-8-ANEPPS varies proportionally to changes in transmembrane voltage. This dye is most suitable for measuring larger changes in membrane voltage, such as the onset of induced membrane voltage in nonexcitable cells exposed to external electric fields or action potentials in excitable cells. Since di-8-ANEPPS stains the membrane, it can also be used simply as a cell membrane marker.
References
[1] GORAZD PUCIHAR Damijan M Tadej Kotnik. Measuring the induced membrane voltage with Di-8-ANEPPS.[J]. Jove-Journal of Visualized Experiments, 2009, 33. DOI:
10.3791/1659
Excitation / Emission Maximum
467 nm/631 nm
