H-Gly-Gly-AMC

Description

Gly-Gly-AMC is a fluorogenic peptide substrate. It has been used to assess the activity of bacterial proteases from P. aeruginosa and S. aureus.¹ Activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm.

References

1. Wildeboer, D., Jeganathan, F., Price, R.G., et al. Characterization of bacterial proteases with a panel of fluorescent peptide substrates Anal. Biochem. 384(2),321-328(2009).