Uses
Methyl tanshinonate is a tanshinone, that can be isolated from Salvia miltiorrhiza (S. miltiorrhiza) Bunge (Lamiaceae). Methyl tanshinonate is a potent inhibitor of Mpro enzyme in SARS-CoV (IC50 = 21.1 μM). Methyl tanshinonate can be used for diabetes and SARS-CoV research[1][2][3].
Synthesis
(1) extraction: divided into two steps, the solvent used can be water or any kind of alcohols, ketones and esters solvents, or a mixture of these solvents in a certain proportion, or from these solvents with acids, alkalis, salts formulated as acidic or alkaline solvents. The extraction method may be decoction, heating and reflux, ultrasonic extraction, cold immersion, percolation, microwave extraction, high-pressure extraction and the like. The preferred extraction process is as follows: extraction in two steps, firstly, Salvia miltiorrhiza herb is added with 50-90% ethanol, refluxed 2 to 3 times, each time for 1 to 2 hours, the amount of solvent is 5 to 15 times the amount (L/kg); secondly, the dregs of the medicine are evaporated to remove the solvent, and then added with 0 to 50% ethanol, refluxed 2 to 3 times, each time for 1 to hours, the amount of solvent is 5 to 15 times the amount (L/kg).
(2) Filtration: the two-step extraction solution was filtered separately. This includes centrifugation, suction filtration, ultrafiltration, pressure filtration, etc., with or without any of the following clarifiers or combinations thereof: alcohol precipitant, gelatin, kaolin, various resins, polyethylene glycol, polyethylenetriol, chitosan, and natural clarifiers of finished products, such as 101 Juice Clarifier, ZTC+1 Natural Clarifier, etc.
(3) Concentration: the two-step extracts are filtered separately, including film evaporation, rotary evaporation and decoction concentration under atmospheric pressure or reduced pressure.
(4) Drying: including vacuum drying, spray drying and freeze drying.
References
[1] Kim DH, et al. Characterization of the inhibitory activity of natural tanshinones from Salvia miltiorrhiza roots on protein tyrosine phosphatase 1B. Chem Biol Interact. 2017 Dec 25;278:65-73. DOI:
10.1016/j.cbi.2017.10.013[2] Ni L, et al. Qualitative analysis of the roots of Salvia miltiorrhiza and Salvia yunnanensis based on NIR, UHPLC and LC-MS-MS. J Pharm Biomed Anal. 2019 Jun 5;170:295-304. DOI:
10.1016/j.jpba.2019.01.010[3] Diniz LRL, et al. Bioactive Terpenes and Their Derivatives as Potential SARS-CoV-2 Proteases Inhibitors from Molecular Modeling Studies. Biomolecules. 2021 Jan 7;11(1):74. DOI:
10.3390/biom11010074
