Uses
MAP4343 is the 3-methylether derivative of Pregnenolone. MAP4343 binds in vitro to microtubule-associated protein 2 (MAP2), stimulates the polymerization of tubulin, enhances the extension of neurites and protects neurons against neurotoxic agents[1][2].
Biological Activity
MAP4343 is a 3-methyl ether derivative of Pregnenolone. It binds to microtubule-associated protein 2 (MAP2) in vitro, stimulates the polymerization of tubulin, enhances neurite extension and protects neurons from neurotoxic agents.
in vitro
MAP4343 (40 μM; 15 or 30 min) stimulates microtubule polymerization in vitro. It stimulates nerve growth factor (NGF)-induced neurite outgrowth in PC12 cells.
in vivo
MAP4343 (12 mg/kg; daily sc for 28 d) improves the recovery of locomotor function after spinal cord injury (SCI) in rats.
MAP4343 (4 mg/kg; daily sc for 6 d) increases MAP2 levels in spinal cord after SCI in rats.
MAP4343 (4 mg/kg; daily sc for 6 d) increases the size of lumbar spinal motoneurons' dendrite arbours after SCI in rats.
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| Animal Model: | Adult Sprague-Dawley male rats with spinal cord injury |
| Dosage: | 12 mg/kg |
| Administration: | Daily sc for 28 days |
| Result: | Increased the number of animals able to walk with weight-supported plantar steps.
Showed forelimb-hindlimb coordination at the end of the study. |
References
[1] Fontaine-Lenoir V, et, al. Microtubule-associated protein 2 (MAP2) is a neurosteroid receptor. Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4711-6. DOI:
10.1073/pnas.0600113103[2] Duchossoy Y, et, al. Treatment of experimental spinal cord injury with 3β-methoxy-pregnenolone. Brain Res. 2011 Jul 27;1403:57-66. DOI:
10.1016/j.brainres.2011.05.065
![1-[(3S,8S,9S,10R,13R,14S,17S)-3-methoxy-10,13-dimethyl-2,3,4,7,8,9,11, 12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-17-yl]ethanone Structure](https://www.chemicalbook.com/CAS/GIF/511-26-2.gif)
