Description
p-APMSF is an irreversible inhibitor of serine proteases with K
i values of 1.02, 1.18, 1.5, and 1.54 μM for bovine trypsin, human thrombin, bovine plasmin, and bovine Factor Xa, respectively. It is selective for these proteases over bovine chymotrypsin and acetylcholinesterase.
Uses
p-APMSF is an irreversible inhibitor of serinase. p-APMSF
has substrate specificity for positively charged side chains of amino acids,
lysine, or arginine. p-APMSF can be used to study the irreversible inhibition
of trypsin and thrombin [1].
Biochem/physiol Actions
Irreversible inhibitor of serine proteases with lysine or arginine substrate specificities. Effective concentration 10-100 μM. Frequently used to characterize newly discovered proteases.
References
[1] Laura
R, et al. (p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor
of serine proteases. Biochemistry. 1980 Oct 14;19(21):4859-64. DOI:
10.1021/bi00562a024
