LIPOPROTEIN LIPASE Struktur

Lipase, Lipoprotein

Bezeichnung:Lipase, Lipoprotein

CAS-Nr9004-02-8

Englisch Name:LIPOPROTEIN LIPASE

CBNumberCB9130935

SummenformelNULL

Molgewicht0

MOL-DateiMol file

Synonyma

Lipase, Lipoprotein

Lipase, Lipoprotein physikalisch-chemischer Eigenschaften

storage temp.	-20°C
Aggregatzustand	powder
Farbe	yellow-brown
Merck	13,5534
EPA chemische Informationen	Lipase, lipoprotein (9004-02-8)

Sicherheit

S-Sätze:	22-24/25-36/37
WGK Germany	3
RTECS-Nr.	TO9776500
F	3-10

Gefahreninformationscode (GHS)

LIPOPROTEIN LIPASE Chemische Eigenschaften,Einsatz,Produktion Methoden

S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
S36/37:Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.
Verwenden
Lipoprotein Lipase from bovine milk has been used:
- as a supplement to test its effect on DiI (1,1′-dioctadecyl-3,3,3′-tetramethyl-indocarbocyanine perchlorate)- very-low-density lipoprotein (VLDL) uptake in breast cancer MDA-MB-231 cells
- to treat human brain microvascular endothelial cells (HBMECs) for the lipolysis of triglyceride-rich lipoproteins (TGRL)
- to test its effect on gene expression in normal human astrocytes
Verwenden
Lipoprotein Lipase from Burkholderia sp. has been used in the subcellular fractionation of mussel Mytilus galloprovincialis to identify the biomolecule attached to cytosolic fraction of okodaic acid. It has also been used to test its effect on serum amyloid A induced granulocyte colony-stimulated factor (G-CSF) expression in response to bacterial lipoprotein.
Allgemeine Beschreibung
Lipoprotein Lipase (LPL) is a glycerol ester hydrolase. Several bacteria that produce LPL, belongs to the genus Pseudomonas, Serratia and Mucor.
Biochem/physiol Actions
Malic dehydrogenase catalyzes the dehydrogenation of L-malate by NAD+.
läuterung methode
Purify the lipase by affinity chromatography on heparin-Sepharose; Ki 0.026mM for very low density lipoprotein. It is inhibited by 2-mercaptoethanol, Cys, Ca, Hg, Mg and Mn ions. Protamine sulfate, 1mg of bovine serum albumin/mL or in 50% glycerol at -70o, stabilises the lipase for several days. 60% loss of activity occurs at 0o/1hour in the presence of 1% of bovine serum albumin. [Shirai et al. Biochim Biophys Acta 665 504 1981.] Lipoproteins (from human plasma). Individual human plasma lipid peaks are removed from plasma by ultracentrifugation; then they are separated and purified by agarose-column chromatography. Fractions are characterised immunologically, chemically, electrophoretically and by electron microscopy. [Rudel et al. Biochem J 13 89 1974.]

Lipase, Lipoprotein Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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