2′,7′-Dichlorodihydrofluorescein Diacetate Struktur

2′,7′-Dichlorodihydrofluorescein Diacetate

Bezeichnung:2′,7′-Dichlorodihydrofluorescein Diacetate

CAS-Nr4091-99-0

Englisch Name:2′,7′-Dichlorodihydrofluorescein Diacetate

CBNumberCB8497619

SummenformelC24H16Cl2O7

Molgewicht487.29

MOL-Datei4091-99-0.mol

2′,7′-Dichlorodihydrofluorescein Diacetate physikalisch-chemischer Eigenschaften

Schmelzpunkt	209-210 °C(lit.)
Siedepunkt	593.5±50.0 °C(Predicted)
Dichte	1.463±0.06 g/cm3(Predicted)
storage temp.	-20°C
Löslichkeit	DMF: soluble
pka	3.79±0.36(Predicted)
Aggregatzustand	Crystalline solid
Farbe	White to off-white
BRN	4610273
Stabilität	Light Sensitive
EPA chemische Informationen	2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	22-24/25-26
WGK Germany	3
F	10-21
HS Code	29329990

Gefahreninformationscode (GHS)

2′,7′-Dichlorodihydrofluorescein Diacetate Chemische Eigenschaften,Einsatz,Produktion Methoden

S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
Beschreibung
DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M⁻¹cm⁻¹).
Chemische Eigenschaften
white to yellow-beige or pale pink powder
Verwenden
2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
Verwenden
2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.
Verwenden
Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.
Allgemeine Beschreibung
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.
Biochem/physiol Actions
Cell permeable: yes
Lager
Store at -20°C

2′,7′-Dichlorodihydrofluorescein Diacetate Upstream-Materialien And Downstream Produkte

2′,7′-Dichlorodihydrofluorescein Diacetate Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

4091-99-0, 2′,7′-Dichlorodihydrofluorescein Diacetate Verwandte Suche:

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