Bis[5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium]sulfat

Bezeichnung:Bis[5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium]sulfat

Englisch Name:CI 51180

CBNumberCB7474347

SummenformelC20H20N3O5S-

Molgewicht414.46

Synonyma

Bis[5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium]sulfat

Bis[5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium]sulfat physikalisch-chemischer Eigenschaften

Schmelzpunkt	>300 °C (dec.)(lit.)
storage temp.	room temp
Löslichkeit	Soluble in water, ethanol
Colour Index	51180
Aggregatzustand	Powder
Farbe	Dark green to black
Säure-Base-Indikators(pH-Indikatoren)	10.1(BLUE)-11.1(RED)
Wasserlöslichkeit	Soluble in ethanol, and water (50 g/L) at 25°C.
maximale Wellenlänge (λmax)	633 nm
Biologische Anwendungen	Detecting microorganisms; treating virus infectious diseases; photodynamic therapy

Sicherheit

Kennzeichnung gefährlicher	Xi
S-Sätze:	22-24/25
WGK Germany	3
RTECS-Nr.	DJ1931000
HS Code	3212900000
Toxizität	mouse,LD,intraperitoneal,> 500mg/kg (500mg/kg),"Summary Tables of Biological Tests," National Research Council Chemical-Biological Coordination Center. Vol. 4, Pg. 46, 1952.

Gefahreninformationscode (GHS)

S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
Chemische Eigenschaften
dark green to black powder
Verwenden
Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.
Verwenden
Nile Blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue
Verwenden
Nile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites.
Allgemeine Beschreibung
Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.
Biochem/physiol Actions
Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.
läuterung methode
Crystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.]