Chapso

Bezeichnung:Chapso

CAS-Nr82473-24-3

Englisch Name:Chapso

CBNumberCB7323821

SummenformelC32H58N2O8S

Molgewicht630.88

MOL-Datei82473-24-3.mol

Chapso physikalisch-chemischer Eigenschaften

Schmelzpunkt	184-186 °C(lit.)
Dichte	1.0453 (rough estimate)
Brechungsindex	1.6900 (estimate)
storage temp.	-20°C
Löslichkeit	H₂O: 10 mg/mL at 20 °C, clear, colorless
Aggregatzustand	White solid
Farbe	White crystalline
Geruch (Odor)	Odorless
Wasserlöslichkeit	10 g/l at 20 °C
Merck	13,2052
BRN	5842642
CAS Datenbank	82473-24-3(CAS DataBase Reference)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	26-36
WGK Germany	3
F	3-10
HS Code	2924297099

Gefahreninformationscode (GHS)

Chapso Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Chemische Eigenschaften
Chapso is a white crystalline powder. It is a zwitterionic detergent similar to Chaps, however, Chapso is more soluble and particularly better for the solubilization of integral membrane proteins.
Verwenden
Chapso is a nondenaturing biological detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins.
Application
3-(cholamidopropyl)(dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO) may be used in bicelle preparation for β2-adrenergic receptor reconstitution.
CHAPSO has been used:
in a study to assess the processes of liposome solubilization and reconstitution of two transport proteins
in a study to investigate a novel bicelle system for orienting macromolecules over a wide temperature range
as a mixture with dilauroyl phosphatidylcholine (DLPC) in solid-state nuclear magnetic resonance (NMR) technique to study membrane-associated proteins
A nondenaturing zwitterionic detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins.
Allgemeine Beschreibung
CHAPSO is a non-denaturing zwitterionic bile salt derivative, derived from Chaps by the addition of a functional hydroxyl group.
läuterung methode
Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.]

Chapso Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

82473-24-3, Chapso Verwandte Suche:

Send Inquriy