CALCEIN-AM Struktur

CALCEIN-AM

Bezeichnung:CALCEIN-AM

CAS-Nr148504-34-1

Englisch Name:CALCEIN-AM

CBNumberCB6304173

SummenformelC46H46N2O23

Molgewicht994.86

MOL-Datei148504-34-1.mol

CALCEIN-AM physikalisch-chemischer Eigenschaften

Schmelzpunkt	>250℃
Siedepunkt	982.7±65.0 °C(Predicted)
Dichte	1.49±0.1 g/cm3(Predicted)
Brechungsindex	n20/D 1.479
Flammpunkt	85 °C
storage temp.	-20°C
Löslichkeit	DMSO: soluble10mg/mL, clear, colorless to slightly yellow
pka	2.66±0.50(Predicted)
Aggregatzustand	powder
Farbe	Colorless
maximale Wellenlänge (λmax)	< 300 nm
Biologische Anwendungen	Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	26-36
RIDADR	NA 1993 / PGIII
WGK Germany	3
F	10-21

CALCEIN-AM Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Verwenden
A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule
Verwenden
Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.
Verwenden
Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions.
Definition
ChEBI: Calcein am is an organic heteropentacyclic compound that is calcein in which all four carboxy group hydrogens have been substituted by (acetyloxy)methoxy groups and the hyrodgens of the two hydroxy groups have been substituted by acetyl groups. It is a a non-fluorescent probe cleaved to a fluorescent probe by non-specific intracellular esterases. It has a role as a fluorochrome. It is an acetate ester, an organic heteropentacyclic compound, a gamma-lactone, an oxaspiro compound, a member of 2-benzofurans and a xanthene dye. It is functionally related to a calcein.
Allgemeine Beschreibung
Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.
Biochem/physiol Actions
Fluorescent cell permeable derivative of calcein.
Lager
-20°C

CALCEIN-AM Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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