RESORUFIN SODIUM SALT Struktur

7-Hydroxy-3H-phenoxazin-3-on, Natriumsalz

Bezeichnung:7-Hydroxy-3H-phenoxazin-3-on, Natriumsalz

CAS-Nr34994-50-8

Englisch Name:RESORUFIN SODIUM SALT

CBNumberCB5471948

SummenformelC12H6NNaO3

Molgewicht235.17

MOL-Datei34994-50-8.mol

Synonyma

7-Hydroxy-3H-phenoxazin-3-on, Natriumsalz

7-Hydroxy-3H-phenoxazin-3-on, Natriumsalz physikalisch-chemischer Eigenschaften

Schmelzpunkt	>300 °C(lit.)
storage temp.	room temp
Löslichkeit	Methanol (Slightly), Water (Slightly)
Aggregatzustand	Solid
Farbe	Dark Purple to Black
BRN	174850

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	26-37-36
WGK Germany	3
RTECS-Nr.	SP7698000

Gefahreninformationscode (GHS)

RESORUFIN SODIUM SALT Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S37:Geeignete Schutzhandschuhe tragen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Chemische Eigenschaften
Dark Purple Solid
Verwenden
Resorufin has been used to determine the cytochrome P450 (CYP) 2A5 coumarin 7-hydroxylase (COH) activity changes over a broad range of lipopolysaccharide (LPS) doses. It has also been used as an internal standard for ethoxyresurufin-o-deethylase (EROD) analysis.
Verwenden
Resorufin is a pink fluorescent dye that is not fluorescent until activated by UV light. Resorufin is an anionic tricyclic dye.
Allgemeine Beschreibung
Resorufin is a fluorescent indicator.
läuterung methode
The free acid is purified by dissolving it in conc H2SO4 which on dilution precipitates resorufin and is filtered off, washed well with hot H2O and dried. [Eichler J Prakt Chem 139 113 1934.] Detailed UV and IR studies were reported by Musso & Mattheis Chem Ber 90 1814 1957]. [pKa: Musso & Rathjen Chem Ber 92 751 1959, Beilstein 27 1V 2263.] The monoacetate [1152-14-3] M 252.2, m 2 2 3o is a fluorogenic substrate used for hydrolytic enzymes, and its fluorescence has Emax 593nm (Eex 500nm, in 0.1M phosphate pH 8.0 with lipase) [Kitson & Kitson Biochem J 322 701 1997]. [Beilstein 27 IV 2263.] The O7-methylresorufin (7-methoxy-3H-phenoxazin-3-one) [5735-89-3] M 227.2, m > 2 2 0o has fluorescence at Emax 585nm (Eexcit 571 nm in deacylase solution) and is used to differentiate isoenzymes of cytochrome P-450. It is insoluble in H2O and dilute alkali but is soluble in EtOH and CHCl3 to give an orange yellow colour. [Kehrmann Justus Liebigs Ann Chem 372 352 1910, Beilstein 27 II 108.]

7-Hydroxy-3H-phenoxazin-3-on, Natriumsalz Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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