3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3',6'-diyldiacetat

Bezeichnung:3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3',6'-diyldiacetat

CAS-Nr596-09-8

Englisch Name:Fluorescein diacetate

CBNumberCB5305799

SummenformelC24H16O7

Molgewicht416.38

MOL-Datei596-09-8.mol

Synonyma

3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3',6'-diyldiacetat

3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3',6'-diyldiacetat physikalisch-chemischer Eigenschaften

Schmelzpunkt	200-203 °C(lit.)
Siedepunkt	494.73°C (rough estimate)
Dichte	1.2738 (rough estimate)
Brechungsindex	1.6810 (estimate)
storage temp.	−20°C
Löslichkeit	acetone: soluble25mg/mL
Aggregatzustand	Crystalline Powder
pka	6.4(at 25℃)
Farbe	Light yellow
Säure-Base-Indikators(pH-Indikatoren)	Weak green uorescence (6.0) to strong green uorescence (7.2)
Wasserlöslichkeit	Soluble in chloroform, DMSO, ethanol, and water.
maximale Wellenlänge (λmax)	490nm
BRN	62575
Major Application	LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia
Biologische Anwendungen	Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease
CAS Datenbank	596-09-8(CAS DataBase Reference)
EPA chemische Informationen	Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	24/25-36/37/39-27-26
WGK Germany	3
TSCA	Yes
HS Code	32129000

Gefahreninformationscode (GHS)

Fluorescein diacetate Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S24/25:Berührung mit den Augen und der Haut vermeiden.
S36/37/39:Bei der Arbeit geeignete Schutzkleidung,Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.
S27:Beschmutzte, getränkte Kleidung sofort ausziehen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Beschreibung
Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields fluorescein.
Chemische Eigenschaften
Light yellow powder
Verwenden
Fluorescein diacetate is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. It has been used for the differentiation of live cells from dead cells. It can be also used as a fluorogenic substrate for hGSTP1-1.
Application
Diacetylfluorescein is used in fluorometric staining in the imaging of mammalian cells. Also used in the synthesis of nanocrystal biolabels with releasable fluorophores used in immunoassays. Dyes and metabolites.
Biotechnological Applications
Fluorescein Diacetate Stain is an esterase substrate that can permeate cell membranes. In viable cells with an active metabolism, intracellular esterases hydrolyze Fluorescein Diacetate Stain to produce the green fluorescent compound fluorescein. Fluorescein accumulates in viable cells, causing them to fluoresce green.
Fluorescein Diacetate Stain can be used with Propidium Iodide Stain for Yeast to assess cell viability with the automated fluorescence cell counters of the LUNA® family. Viable cells convert fluorescein diacetate into fluorescein and thus fluoresce green. Nonviable cells take up propidium iodide and fluoresce red.
Fluorescence Signal Enhancer 1 can be used with Fluorescein Diacetate Stain to inhibit ATP-dependent fluorescein efflux from metabolically active cells. This will result in lower background and increased signal intensity, thereby enhancing the fluorescein signal-to-noise ratio.

3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3',6'-diyldiacetat Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

596-09-8, Fluorescein diacetate Verwandte Suche:

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