Synthetase, Acetyl-Coenzym A

Acetyl-CoA synthetase Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO₂ fixation. It may also be used in gene expression studies.

• Biochem/physiol Actions

  Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol .

• läuterung methode

  This enzyme has been purified by extraction into phosphate buffer pH 6.8-7.0 containing 2-mercaptoethanol and EDTA, protamine sulfate precipitation, polyethylene glycol fractionation, Alumina  gel filtration, concentration by (NH4)2SO4 precipitation, Bio-Gel A-0.5m chromatography and DEAE-cellulose gradient chromatography. It has Km (apparent) 0.24mM (for acetate) and 0.035mM (for CoA); 1.2 mM for ATP and Mg2+ 4.0mM. [Frenkel & Kitchens Methods Enzymol 71 317 1981.]

Synthetase, Acetyl-Coenzym A Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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