DNA polymerase Struktur

Nucleotidyltransferase, Deoxyribonucleat

Bezeichnung:Nucleotidyltransferase, Deoxyribonucleat

CAS-Nr9012-90-2

Englisch Name:DNA polymerase

CBNumberCB3113594

SummenformelNULL

Molgewicht0

MOL-DateiMol file

Synonyma

Nucleotidyltransferase, Deoxyribonucleat

Nucleotidyltransferase, Deoxyribonucleat physikalisch-chemischer Eigenschaften

storage temp.	-20°C
Aggregatzustand	buffered aqueous glycerol solution
Farbe	colorless
EPA chemische Informationen	Nucleotidyltransferase, deoxyribonucleate (9012-90-2)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38-43
S-Sätze:	26-36-24/25-36/37
WGK Germany	3
F	10
TSCA	Yes

Gefahreninformationscode (GHS)

DNA polymerase Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
Verwenden
REDTaq^? Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.
Verwenden
Taq DNA Polymerase from Thermus aquaticus has been used in the process of DNA extraction (during gene amplification and sequencing). It has been used in genotyping. It has also been used in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8).
Verwenden
For routine PCR amplifications REDTaq^? DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis.
Allgemeine Beschreibung
Klenow enzyme is the large, C-terminal fragment (M_r 76,000) of E.coli DNA polymerase I, which can be obtained by subtilisin treatment of intact DNA polymerase I. It retains the 5′→3′ polymerase and the 3′→5′ exonuclease activities of intact DNA polymerase I, but lacks the 5′→3′ exonuclease activity of the native enzyme. The enzyme catalyzes the addition of mononucleotides from deoxynucleoside-5′-triphosphates to the 3′-hydroxyl terminus of a primer/template DNA. This property is used to synthesize DNA complementary to single-stranded DNA templates.
Klenow Enzyme is formed by subtilisin treatment of DNA polymerase I.
Biochem/physiol Actions
Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Nucleotidyltransferase, Deoxyribonucleat Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

9012-90-2, DNA polymerase Verwandte Suche:

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