Hydratase, Phosphopyruvat

ENOLASE Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.

• Definition

  An enzyme active in glycolysis that catalyzes the conversion of 2-phosphoglyceric acid to the phosphorylated enol of pyruvic acid.

• Allgemeine Beschreibung

  Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg²⁺ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes.

• Biochem/physiol Actions

  Enolase plays a key role in glycolysis and gluconeogenesis. Enolase, found in the nucleus of mammalian cells plays a key role in transcriptional modulation of genes, which participates in morphological transformation and cell proliferation.

Hydratase, Phosphopyruvat Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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