3,6-Bis(dimethylamino)xanthyliumchlorid

Bezeichnung:3,6-Bis(dimethylamino)xanthyliumchlorid

CAS-Nr92-32-0

Englisch Name:PYRONIN Y

CBNumberCB1418608

SummenformelC17H19ClN2O

Molgewicht302.8

MOL-Datei92-32-0.mol

Synonyma

3,6-Bis(dimethylamino)xanthyliumchlorid

3,6-Bis(dimethylamino)xanthyliumchlorid physikalisch-chemischer Eigenschaften

Schmelzpunkt	250-260 °C(lit.)
storage temp.	room temp
Löslichkeit	Soluble in water; sparingly soluble in ethanol, ethylene glycol, methyl cellosolve
Aggregatzustand	Powder/Solid
Colour Index	45005
Farbe	Pink
Wasserlöslichkeit	Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).
maximale Wellenlänge (λmax)	548 nm
Merck	13,8097
BRN	3795789
Stabilität	Stable. Incompatible with strong oxidizing agents.
Biologische Anwendungen	Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides
InChIKey	INCIMLINXXICKS-UHFFFAOYSA-M
CAS Datenbank	92-32-0
EPA chemische Informationen	Pyronine Y (92-32-0)

Sicherheit

Kennzeichnung gefährlicher	Xn,Xi
R-Sätze:	68-36/37/38
S-Sätze:	22-24/25-36-26
WGK Germany	3
RTECS-Nr.	BQ1450000
TSCA	Yes
HS Code	32041300

Gefahreninformationscode (GHS)

PYRONIN Y Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R68:Irreversibler Schaden möglich.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Chemische Eigenschaften
green crystalline solid
Verwenden
Pyronin Y has been used:
- in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
- as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
- staining of CD34⁺ cells
Verwenden
Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
Verwenden
Bacterial and biological stain.
Definition
ChEBI: An organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna Pappenheim method.
Allgemeine Beschreibung
Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.
Biochem/physiol Actions
Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.
Lager
Room temperature
läuterung methode
Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.]

3,6-Bis(dimethylamino)xanthyliumchlorid Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

92-32-0, PYRONIN Y Verwandte Suche:

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