补骨脂乙素

生物活性靶点体外研究体内研究补骨脂乙素试剂级价格

补骨脂乙素

CAS号:20784-50-3
英文名:ISOBAVACHALCONE
中文名:补骨脂乙素
CBNumber:CB91412753
分子式:C20H20O4
分子量:324.37
MOL File:20784-50-3.mol

补骨脂乙素化学性质

熔点 :156.8-157.8 °C(Solv: ethyl acetate (141-78-6); hexane (110-54-3))
沸点 :549.0±50.0 °C(Predicted)
密度 :1.243±0.06 g/cm3(Predicted)
储存条件 :Keep in dark place,Inert atmosphere,Store in freezer, under -20°C
溶解度 :Soluble in DMSO
形态 :Yellow oil.
酸度系数(pKa) :7.99±0.40(Predicted)
颜色 :Yellow-orange

补骨脂乙素性质、用途与生产工艺

生物活性 Isobavachalcone (Corylifolinin) 来源于补骨脂，是一种 Akt 信号通路的有效抑制剂，可诱导人类癌细胞凋亡 (以 IC50 值为 7.92 μM 抑制 OVCAR-8 癌细胞生长)，具有抗癌和抗增殖活性。Isobavachalcone 还能诱导 OVCAR-8 细胞中活性氧的产生。
靶点

IC50: 7.92 μM (OVCAR-8 cell)

体外研究

Isobavachalcone (6.0-48.0 μM; 72 hours; OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells) treatment inhibits the proliferation of human cancer cells. Isobavachalcone inhibits PC3, A549, MCF-7, L-02 and HUVEC cells growth with IC50 values of 15.06 μM, 32.2 μM, 28.29 μM, 31.61 μM and 31.3 μM, respectively.
Isobavachalcone (0-18 μM; 6 hours; OVCAR-8 and PC3 cells) treatment results in a concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt and GSK3b phosphorylation.
Isobavachalcone (0-18 μM; 72 hours; OVCAR-8 and PC3 cells) treatment causes apoptosis via caspase- and ROS-involved mitochondrial pathway.

Cell Proliferation Assay

Cell Line:	OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells
Concentration:	6.0-48.0 μM
Incubation Time:	72 hours
Result:	Inhibited the proliferation of human cancer cells.

Western Blot Analysis

Cell Line:	OVCAR-8 or PC3 cells
Concentration:	0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:	6 hours
Result:	A concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt. GSK3b phosphorylation was also inhibited in a concentration- and time-dependent manner.

Apoptosis Analysis

Cell Line:	OVCAR-8 cells and PC3 cells
Concentration:	0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:	72 hours
Result:	Led to dose dependent increase of apoptosis.

体内研究

Isobavachalcone (20 mg/kg; intraperitoneal injection; for 0.5-24 hours; female Kunming mice) treatment results in an increase in blood glucose levels, reaching a maximum within 1 hour and maintaining until 4 hours post-dosing.

Animal Model:	Seven-week-old specific pathogen-free female Kunming mice (18-22 g)
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; for 0.5, 1, 2, 4, 6, 8, 12, 24 hours
Result:	Increased in blood glucose levels.